You have no items in your shopping cart.
Cart summary
Item 1 of 4
Item 1 of 4
Anti-Hu CD222 Purified
Catalog Number: orb402817
| Catalog Number | orb402817 |
|---|---|
| Category | Antibodies |
| Description | Mouse monoclonal antibody to CD222 |
| Target | CD222 |
| Clonality | Monoclonal |
| Isotype | Mouse IgG1 |
| Conjugation | Unconjugated |
| Reactivity | Human |
| Concentration | 1 mg/ml |
| Buffer/Preservatives | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Purity | Purified by protein-A affinity chromatography. |
| Immunogen | Recombinant Vaccinia virus encoding CD222. |
| UniProt ID | P11717 |
| Tested applications | FC, IP, WB |
| Dilution range | Flow cytometry: Extracellular and intracellular staining. Recommended dilution: 2-6 μg/ml. Western blotting: non-reducing conditions. Recommended dilution: 1-2 μg/ml. |
| Application notes | Flow cytometry: Extracellular and intracellular staining. Recommended dilution: 2-6 μg/ml. |
| Antibody Type | Primary Antibody |
| Clone Number | MEM-240 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | Anti-IGF2R antibody, anti-MPR1 antibody, anti-CIMP Read more... |
| Note | For research use only |
| Entrez | 3482 |
Flow cytometry detection of CD222 in human peripheral blood with anti-CD222 (MEM-240) purified, GAM-APC.
Separation of human neutrophil granulocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD222 (MEM-240) purified antibody (concentration in sample 3 µg/ml) GAM APC.
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD222 (MEM-240) purified antibody (concentration in sample 3 µg/ml) GAM APC.
Anti-Hu CD222 Purified (clone MEM-240) works in WB application under non-reducing conditions. Western blotting analysis was performed on whole cell extracts (RIPA lysis buffer) of Jurkat, K562, Raji, and HeLa cell lines, mixed and heated (100°C, 5 min) with reducing and non-reducing SDS-loading buffer. Samples were resolved using 7% Tris-glycine SDS gel electrophoresis. Nitrocellulose membrane blot was probed with mouse IgG1 monoclonal antibody MEM-240 (1 µg/ml), followed by IRDye 800CW Goat-anti-Mouse IgG (green). Mouse anti-GAPDH monoclonal antibody FF26A conjugated with DyLight 680 (0.1 µg/ml), was used as the loading control (red). Multiplex fluorescent Western blot detection was performed. CD222 molecules were detected at ~250 kDa in all analysed cell lines.
- Item 1 of 3
