Anti-Hu CD222 Purified

• Catalog Number: orb402817
• Category: Antibodies
• Description: Mouse monoclonal antibody to CD222
• Clonality: Monoclonal
• Clone Number: MEM-240
• Tested applications: FC, IP, WB
• Reactivity: Human
• Isotype: Mouse IgG1
• Immunogen: Recombinant Vaccinia virus encoding CD222.
• Antibody Type: Primary Antibody
• Concentration: 1 mg/ml
• Dilution range: Flow cytometry: Extracellular and intracellular staining. Recommended dilution: 2-6 μg/ml. Western blotting: non-reducing conditions. Recommended dilution: 1-2 μg/ml.
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: CD222
• Entrez: 3482
• UniProt ID: P11717
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-IGF2R antibody, anti-MPR1 antibody, anti-CIMP
• Note: For research use only
• Application notes: Flow cytometry: Extracellular and intracellular staining. Recommended dilution: 2-6 μg/ml.
• Expiration Date: 12 months from date of receipt.

Flow cytometry detection of CD222 in human peripheral blood with anti-CD222 (MEM-240) purified, GAM-APC.

Separation of human neutrophil granulocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD222 (MEM-240) purified antibody (concentration in sample 3 µg/ml) GAM APC.

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD222 (MEM-240) purified antibody (concentration in sample 3 µg/ml) GAM APC.

Anti-Hu CD222 Purified (clone MEM-240) works in WB application under non-reducing conditions. Western blotting analysis was performed on whole cell extracts (RIPA lysis buffer) of Jurkat, K562, Raji, and HeLa cell lines, mixed and heated (100°C, 5 min) with reducing and non-reducing SDS-loading buffer. Samples were resolved using 7% Tris-glycine SDS gel electrophoresis. Nitrocellulose membrane blot was probed with mouse IgG1 monoclonal antibody MEM-240 (1 µg/ml), followed by IRDye 800CW Goat-anti-Mouse IgG (green). Mouse anti-GAPDH monoclonal antibody FF26A conjugated with DyLight 680 (0.1 µg/ml), was used as the loading control (red). Multiplex fluorescent Western blot detection was performed. CD222 molecules were detected at ~250 kDa in all analysed cell lines.