IGF1 Antibody

• Catalog Number: orb1539160
• Category: Antibodies
• Description: IGF1 Antibody
• Target: IGF1
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Concentration: 0.5 mg/ml
• Buffer/Preservatives: TBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin
• Immunogen: Peptide with sequence C-RSVRAQRHTD, from the internal region of the protein sequence according to NP_001104753.1NP_001104754.1NP_001104755.1NP_000609.1.
• Tested applications: ELISA, IF, IHC, IHC-P, WB
• Dilution range: IF (10 µg/ml), IHC, IHC-P (3.75 µg/ml), Peptide-ELISA (1:32000), WB (0.5 - 2 µg/ml)
• Application notes: Further information: Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 15kD band observed in Human Uterus lysates (calculated MW of 15.2kD according to NP_001104754.1). Recommended concentration 0.5-2 ug/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: IGF1, IGF-IA, IGF-IB, IGFI, IBP1, IGF-I, Insulin-l
• Note: For research use only

IGF1 Antibody Immunofluorescence analysis of paraformaldehyde fixed HEK293 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).

IGF1 antibody (0.5 µg/ml) staining of Human Uterus lysate (35 µg protein in RIPA buffer). Detected by chemiluminescence.

IGF1 Antibody Flow cytometric analysis of paraformaldehyde fixed A549 cells (blue line), permeabilized with 0.5% Triton.