IGF1 Antibody

SKU: orb125044

Description

Goat Polyclonal to IGF1. NP_001104753.1; NP_001104754.1; NP_001104755.1; NP_000609.1, PMID: 20230795 and GeneID: 3479.

Images & Validation

• Tested Applications: ELISA, FC, IF, IHC, WB
• Dilution range: ELISA: 1:32000, WB: 0.5-2 μg/ml, IHC-P: 3.75 μg/ml
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Porcine, Rat
• Application Notes:
  WB: Approx 15kDa band observed in Human Uterus lysates (calculated MW of 15.2kDa according to NP_001104754.1). Recommended concentration 0.5-2µg/ml. Primary incubation 1 hour at room temperature. Negative Control: HepG2 cell lysate. Prelimi. IHC: Paraffin embedded Human Spleen. Recommended concentration: 3.75µg/ml.. Notes: Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HEK293 cells. Recommended concentration: 10µg/ml. Flow Cytometry: Flow cytometric analysis of A549 cells. Recommended concentration: 10ug/ml

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: IGF1
• Molecular Weight: 17.8; 15.2; 21.8; 17.0
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

anti IGF1 antibody, anti insulin-like growth factor 1 (somatomedin C) antibody, anti IGF1A antibody, anti IGFI antibody, anti OTTHUMP00000195084 antibody, anti insulin-like growth factor 1 antibody

Immunohistochemical staining of Human Spleen using IGF1 antibody

Western blot analysis of Human Uterus lysate using IGF1 antibody

Flow cytometric analysis of paraformaldehyde fixed A549 cells using IGF1 antibody

Immunofluorescence analysis of HEK293 cells using IGF1 antibody

Quick Database Links

Gene Symbol

IGF1

NCBI Gene ID

3479

References

Wang, Yan et al. Rumen-Protected Glucose Stimulates the Insulin-Like Growth Factor System and mTOR/AKT Pathway in the Endometrium of Early Postpartum Dairy Cows Animals (Basel), 10, E357 (2020)

PubMed: 32102173