IGF1 Antibody

SKU: orb1249042

Description

IGF1 Antibody

Images & Validation

• Tested Applications: ELISA, FC, IF, IHC, WB
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Porcine, Rat
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 15kDa band observed in Human Uterus lysates (calculated MW of 15.2kDa according to NP_001104754.1). Recommended concentration 0.5-2µg/mL. Primary incubation 1 hour at room temperature. Negative Control: HepG2 cell lysate. Preliminary testing was unsuccessful on Mouse and Rat for this particular batch.Immunohistochemistry:Paraffin embedded Human Spleen. Recommended concentration: 3.75µg/mL.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HEK293 cells. Recommended concentration: 10µg/mL. Flow Cytometry: Flow cytometric analysis of A549 cells. Recommended concentration: 10µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-RSVRAQRHTD
• Target: IGF1
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

IGF1, insulin-like growth factor 1 (somatomedin C), IGF1A, IGFI, OTTHUMP00000195084, insulin-like growth factor 1

orb1249042 (0.5 ug/ml) staining of Human Uterus (A) and negative control HepG2 (B) lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence

orb1249042 (3.75 ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1249042 Immunofluorescence analysis of paraformaldehyde fixed HEK293 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI.

orb1249042 Flow cytometric analysis of paraformaldehyde fixed A549 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line).

Quick Database Links

Gene Symbol

IGF1

UniProt

P05019

RefSeq (Protein):NP_001104754.1, NP_001104753.1, NP_000609.1, NP_001104755.1