IGF1 Antibody

• Catalog Number: orb1249042
• Category: Antibodies
• Description: IGF1 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IF, IHC, WB
• Predicted Reactivity: Bovine, Canine, Mouse, Porcine, Rat
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-RSVRAQRHTD
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 15kDa band observed in Human Uterus lysates (calculated MW of 15.2kDa according to NP_001104754.1). Recommended concentration 0.5-2ug/ml. Primary incubation 1 hour at room temperature. Negative Control: HepG2 cell lysate. Preliminary testing was unsuccessful on Mouse and Rat for this particular batch.Immunohistochemistry:Paraffin embedded Human Spleen. Recommended concentration: 3.75ug/ml.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HEK293 cells. Recommended concentration: 10ug/ml. Flow Cytometry: Flow cytometric analysis of A549 cells. Recommended concentration: 10ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: IGF1
• UniProt ID: P05019
• NCBI: NP_000609.1, NP_001104753.1, NP_001104754.1, NP_001104755.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: IGF1, insulin-like growth factor 1 (somatomedin C)
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 15kDa band observed in Human Uterus lysates (calculated MW of 15.2kDa according to NP_001104754.1). Recommended concentration 0.5-2ug/ml. Primary incubation 1 hour at room temperature. Negative Control: HepG2 cell lysate. Preliminary testing was unsuccessful on Mouse and Rat for this particular batch.Immunohistochemistry:Paraffin embedded Human Spleen. Recommended concentration: 3.75ug/ml.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HEK293 cells. Recommended concentration: 10ug/ml. Flow Cytometry: Flow cytometric analysis of A549 cells. Recommended concentration: 10ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1249042 (0.5 ug/ml) staining of Human Uterus (A) and negative control HepG2 (B) lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence

orb1249042 (3.75 ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1249042 Immunofluorescence analysis of paraformaldehyde fixed HEK293 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI.

orb1249042 Flow cytometric analysis of paraformaldehyde fixed A549 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line).