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Catalog Number | orb1247371 |
---|---|
Category | Antibodies |
Description | IFT88 Antibody |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, FC, IF, IHC, WB |
Predicted Reactivity | Canine, Rat |
Reactivity | Human, Mouse |
Immunogen | The immunogen for this antibody is: C-KKRIDEDDFADEE |
Concentration | 500 ug/mL |
Dilution range | Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 85-90kDa band observed in lysates of cell lines HepG2 and NIH3T3 and in Human Kidney lysates (calculated MW of 85.3kDa according to Human NP_001340507.1 and 90.4kDa according to NP_001340498.1, and 93.1kDa according to Mouse NP_033402.2 93. and 89.2kDa according to XP_006518873.1). Recommended concentration: 0.3-1ug/ml. Primary incubation 1 hour at room temperature.Immunohistochemistry:In paraffin embedded Human Kidney shows staining of the brush border in PCT. Recommended concentration: 3ug/ml.Immunofluorescence: Expression of the protein seen within the nucleus and cytoplasm of HepG2 cells. Recommended concentration: 10ug/ml. This antibody has been successfully used in IF on Mouse Olfactory and Respiratory Epithelium cells as described in the following paper: Miyoshi et al, FASEB J. 2009 Oct;23(10):3289-97, PMID: 19470799. Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 10ug/ml. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Target | IFT88 |
UniProt ID | Q13099 |
NCBI | NP_006522.2, NP_001340507.1, NP_001340498.1, NP_783195.2 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | IFT88, polaris, TTC10, tetratricopeptide repeat do Read more... |
Note | For research use only |
Application notes | Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 85-90kDa band observed in lysates of cell lines HepG2 and NIH3T3 and in Human Kidney lysates (calculated MW of 85.3kDa according to Human NP_001340507.1 and 90.4kDa according to NP_001340498.1, and 93.1kDa according to Mouse NP_033402.2 93. and 89.2kDa according to XP_006518873.1). Recommended concentration: 0.3-1ug/ml. Primary incubation 1 hour at room temperature.Immunohistochemistry:In paraffin embedded Human Kidney shows staining of the brush border in PCT. Recommended concentration: 3ug/ml.Immunofluorescence: Expression of the protein seen within the nucleus and cytoplasm of HepG2 cells. Recommended concentration: 10ug/ml. This antibody has been successfully used in IF on Mouse Olfactory and Respiratory Epithelium cells as described in the following paper: Miyoshi et al, FASEB J. 2009 Oct;23(10):3289-97, PMID: 19470799. Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 10ug/ml. |
Expiration Date | 12 months from date of receipt. |
orb1247371 (3 ug/ml) staining of paraffin embedded Human Kidney. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
orb1247371 (0.5 ug/ml) staining of Human Kidney lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.
orb1247371 (0.5 ug/ml) staining of HepG2 (A) and NIH3T3 (B) cell lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.
orb1247371 Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing nuclear and cytoplasmic staining.
orb1247371 Flow cytometric analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line).
ELISA, FC, ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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