IFT88/Polaris Antibody

SKU: orb19151

Description

Goat polyclonal antibody to IFT88

Images & Validation

• Tested Applications: ELISA, IF, WB
• Dilution range: ELISA: 1:128000, WB: 1-3ug/ml, IHC-P: 10ug/ml
• Reactivity: Human, Mouse
• Predicted Reactivity: Canine, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Kidney shows staining of the brush border in PCT. Recommended concentration, 3ug/ml.WB: Preliminary experiments in Human Kidney, Liver and Lung lysates gave no specific signal but low background (at antibody concentration up to 1 μg/ml). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: IFT88 / Polaris
• Molecular Weight: 94.3; 93.2
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

anti IFT88 antibody, anti polaris antibody, anti TTC10 antibody, anti tetratricopeptide repeat domain 10 antibody, anti HGNC:20606 antibody, anti D13S1056E antibody, anti DAF19 antibody, anti IFT88 antibody, anti MGC26259 antibody, anti RP11-172H24.2 antibody, anti TG737 antibody, anti hTg737 antibody, anti TPR repeat protein 10 antibody, anti Probe hTg737 (polycystic kidney disease, autosomal recessive) antibody, anti Tg737 protein antibody, anti intraflagellar transport 88 homolog antibody, anti recessive polycystic kidney disease protein Tg737 homolog antibody, anti intraflagellar transport 88 homolog (Chlamydomonas) antibody

2 µg/ml staining of K562 (A), and 3 ug/ml THP-1 (B), U251 (C) and KNRK (D) cell lysate (35 µg protein in RIPA buffer). Detected by chemiluminescence.

Immunofluorescence analysis of paraformaldehyde fixed A549 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).

Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).

Quick Database Links

Gene Symbol

IFT88 / Polaris

NCBI Gene ID

8100