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Goat anti-IFT88 / Polaris Antibody

Catalog Number: orb19151

DispatchUsually dispatched within 5-10 working days
$ 500.00
Catalog Numberorb19151
CategoryAntibodies
DescriptionGoat polyclonal antibody to IFT88
Species/HostGoat
ClonalityPolyclonal
Tested applicationsELISA, IF, WB
ReactivityCanine, Human, Mouse, Rat
Dilution rangeELISA: 1:128000, WB: 1-3ug/ml, IHC-P: 10ug/ml
ConjugationUnconjugated
MW94.3; 93.2
TargetIFT88 / Polaris
Entrez8100
Protein SequenceKKRIDEDDFADEE
RRIDAB_10751672
StorageAliquot and store at -20°C. Minimize freezing and thawing.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Alternative namesanti IFT88 antibody, anti polaris antibody, anti T
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NoteFor research use only
Application notesELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Kidney shows staining of the brush border in PCT. Recommended concentration, 3ug/ml.WB: Preliminary experiments in Human Kidney, Liver and Lung lysates gave no specific signal but low background (at antibody concentration up to 1 μg/ml). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates
Expiration Date12 months from date of receipt.
Goat anti-IFT88 / Polaris Antibody

2 µg/ml staining of K562 (A), and 3 ug/ml THP-1 (B), U251 (C) and KNRK (D) cell lysate (35 µg protein in RIPA buffer). Detected by chemiluminescence.

Goat anti-IFT88 / Polaris Antibody

Immunofluorescence analysis of paraformaldehyde fixed A549 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).

Goat anti-IFT88 / Polaris Antibody

Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).