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Catalog Number | orb19151 |
---|---|
Category | Antibodies |
Description | Goat polyclonal antibody to IFT88 |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, IF, WB |
Reactivity | Canine, Human, Mouse, Rat |
Dilution range | ELISA: 1:128000, WB: 1-3ug/ml, IHC-P: 10ug/ml |
Conjugation | Unconjugated |
MW | 94.3; 93.2 |
Target | IFT88 / Polaris |
Entrez | 8100 |
Protein Sequence | KKRIDEDDFADEE |
RRID | AB_10751672 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | anti IFT88 antibody, anti polaris antibody, anti T Read more... |
Note | For research use only |
Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Kidney shows staining of the brush border in PCT. Recommended concentration, 3ug/ml.WB: Preliminary experiments in Human Kidney, Liver and Lung lysates gave no specific signal but low background (at antibody concentration up to 1 μg/ml). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates |
Expiration Date | 12 months from date of receipt. |
2 µg/ml staining of K562 (A), and 3 ug/ml THP-1 (B), U251 (C) and KNRK (D) cell lysate (35 µg protein in RIPA buffer). Detected by chemiluminescence.
Immunofluorescence analysis of paraformaldehyde fixed A549 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).
Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).