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Item 1 of 3
Goat anti-IFT88 / Polaris Antibody
Catalog Number: orb19151
| Catalog Number | orb19151 |
|---|---|
| Category | Antibodies |
| Description | Goat polyclonal antibody to IFT88 |
| Target | IFT88 / Polaris |
| Clonality | Polyclonal |
| Species/Host | Goat |
| Conjugation | Unconjugated |
| Reactivity | Canine, Human, Mouse, Rat |
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Protein Sequence | KKRIDEDDFADEE |
| RRID | AB_10751672 |
| MW | 94.3; 93.2 |
| Tested applications | ELISA, IF, WB |
| Dilution range | ELISA: 1:128000, WB: 1-3ug/ml, IHC-P: 10ug/ml |
| Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Kidney shows staining of the brush border in PCT. Recommended concentration, 3ug/ml.WB: Preliminary experiments in Human Kidney, Liver and Lung lysates gave no specific signal but low background (at antibody concentration up to 1 μg/ml). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates |
| Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
| Alternative names | anti IFT88 antibody, anti polaris antibody, anti T Read more... |
| Note | For research use only |
| Entrez | 8100 |
2 µg/ml staining of K562 (A), and 3 ug/ml THP-1 (B), U251 (C) and KNRK (D) cell lysate (35 µg protein in RIPA buffer). Detected by chemiluminescence.
Immunofluorescence analysis of paraformaldehyde fixed A549 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).
Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).
