IFN-gamma Antibody

• Catalog Number: orb1272507
• Category: Antibodies
• Description: IFN-gamma Antibody
• Target: Ifng
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Mouse
• Form/Appearance: Lyophilized
• Concentration: batch dependent
• Purification: Anti-mIFN-gamma specific antibody was purified by affinity chromatography employing immobilized mIFN-gamma matrix.
• Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mIFN-gamma (murine Interferon-gamma).
• UniProt ID: P01580
• Tested applications: ELISA, NeA, WB
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Ifg, IFN-gInterferon gammaIFN-gamma
• Note: For research use only
• NCBI: P01580

To detect Murine IFN-gamma by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Anti-Murine IFN-gamma (orb1272506) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Murine IFN-gamma.

To detect Murine IFN-gamma by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Murine IFN-gamma is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.