Idoxuridine

SKU: orb1310222

Description

Idoxuridine

Research Area

Infectious Disease & Virology, Metabolism Research

Key Properties

• CAS Number: 54-42-2
• MW: 354.1
• Purity: 99.62%
• Formula: C₉H₁₁IN₂O₅
• SMILES: OCC1OC(CC1O)n1cc(I)c(=O)[nH]c1=O
• Target: Phosphatase,Virus Protease,HSV,Antifection
• Solubility: Ethanol:< 1 mg/mL (insoluble or slightly soluble);DMSO:45 mg/mL (127.08 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (5.65 mM)

Bioactivity

• Target IC50:
  FHV-1:4.3 μM
• In Vitro:
  Idoxuridine is a nucleoside analogue, a modified form of deoxyuridine, similar enough to be incorporated into viral DNA replication, bu the iodine atom added to the uracil component blocks base pairing.Idoxuridine is used for topical treatment of herpetic eye infections due to herpes simplex virus (HSV.
• Cell Research:
  The CRFK cells are cultured in 25 cm2 flasks that contained DMEM with 10% FBS the concentration of idoxuridine is 5, 10, and 50 μM. Cells from 1 control flask and 1 flask containing Each drug concentration are examined by use of an inverted microscope to detect morphologic Changes and evaluate confluence, and cells are then Harvested at 24, 48, and 72 hours. Culture medium is decanted, an the monolayer is rinsed once with PBS solution. Cells are then enzymatically detached with trypsin-EDTA solution. Trypsinization is stopped b the addition of DMEM with 10% FBS, and cells are forcefully pipetted to ensure complete detachment fro the flasks. Each flask is then rinsed once with DMEM with 10% FBS, an the eluent is combined wit the first cell suspension to ensure collection of as many cells as possible from Each flask. Cell-containing medium is centrifuged (300 X g for 6 minutes), an the supernatant is decanted. Cells are then resuspended in a known volume of DMEM and counted on a hemacytometer. Cells cultured in Each drug concentration at Each time point are counted twice, an the total number of cells in Each flask is calculated. (Only for Reference)

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Disclaimer: For research use only

Alternative Names

Phosphatase, Phosphorylases inhibitor, SKF 14287, SKF14287, SKF-14287, DNA polymerase, Dendrid, 5-Iodo-2′-deoxyuridine, 5-IUdR, Antiviral agents, inhibit, Iodinated thymidine analogue, NSC 39661, NSC-39661, NSC39661, Orthopoxvirus, Inhibitor, IDU, IdUrd, Idoxuridine, Herpesviruses, FHV-1, Feline herpesvirus, HSV

Compound Identifiers

PubChem CID

3687