ICAM1 Antibody / CD54

• Catalog Number: orb1151644
• Category: Antibodies
• Description: Mouse monoclonal antibody to ICAM1
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG1
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Purity: Protein A/G affinity
• Immunogen: Recombinant full-length human ICAM1 protein was used as the immunogen for the ICAM1 antibody. The epitope is is known to be in the extracellular domain.
• UniProt ID: P05362
• Tested applications: FACS, IF, IHC-P
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
• Application notes: Optimal dilution of the ICAM1 antibody should be determined by the researcher.
• Antibody Type: Primary Antibody
• Clone Number: ICAM1/6319
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Storage: Aliquot the ICAM1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Hazard Information: This ICAM1 antibody is available for research use only.
• Note: For research use only

IHC staining of FFPE human tonsil tissue with ICAM1 antibody (clone ICAM1/6319) at 2 ug/ml. Negative control inset: PBS instead of primary antibody to control for secondary binding. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human lymph node tissue with ICAM1 antibody (clone ICAM1/6319). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human lymph node tissue with ICAM1 antibody (clone ICAM1/6319). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human renal cell carcinoma tissue with ICAM-1 antibody (clone ICAM1/6319). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.