ICAM-1/CD54 Antibody

SKU: orb1538056

Description

ICAM-1/CD54 Antibody

Images & Validation

• Tested Applications: FC, IHC, IHC-Fr, IHC-P, IP
• Dilution Range: Flo (1:50 - 1:100), IHC, IHC-Fr (1:50 - 1:100), IHC-P (10 µg/ml), IP
• Reactivity: Human
• Application Notes:
  Further information: Immunohistochemistry: This was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration was determined to be 10 ug/ml. Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100ul.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: 15.2
• Immunogen: Human monocytes
• Target: ICAM-1 / CD54
• Purification: Protein G purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS, 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ICAM1, BB2, CD54 antigen, CD54, ICAM-1, p3.58

Quick Database Links

Gene Symbol

ICAM-1 / CD54