PPP1R13L Antibody

SKU: orb345545

Description

iASSP antibody

Research Area

Cell Biology

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:40,000 - 1:160,000, IHC: 1:1,000 - 1:5,000, WB: 1:1,000 - 1:5,000
• Reactivity: Human
• Application Notes:
  This affinity purified antibody has been tested for use in ELISA, immunohistochemistry and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect bands approximately 100 kDa and 50 kDa in size corresponding to isoforms 1 and 2 respectively of iASPP protein by western blotting in the appropriate cell lysate or extract.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 775-800 of human iASPP (isoform 1) protein.
• Target: PPP1R13L
• Purity: This affinity-purified antibody is directed against the human iASPP protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross reactivity with iASPP proteins from human, dog and mouse sources. Expect reactivity against both isoform 1 and isoform 2. Partial reactivity may occur against iASPP from bovine. Reactivity against homologues from other sources is not known.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-iASPP antibody, inhibitor of apoptosis stimulating protein of p53 antibody, Inhibitor of ASPP protein antibody, RelA-associated inhibitor, NFkB interacting protein 1 antibody, Protein iASPP, NKIP1 antibody, PPP1R13B-like protein antibody, IASPP, NKIP1, PPP1R13BL, RAI, PPP1R13L

Biorbyt's Affinity Purified anti-iASPP antibody shows strong cytoplasmic and membranous staining of myocytes in human heart tissue. Tissue was formalin-fixed and paraffin embedded. Brown color indicates presence of protein, blue color shows cell nuclei.

Western blot using Biorbyt's affinity purified anti-iASPP antibody shows detection of a band at ~100 kDa (arrowhead) corresponding to isoform 1 of iASPP in MCF7 whole cell lysates (p/n orb348664). Preincubation with immunizing peptide blocks specific band staining (data not shown). Approximately 35 µg of lysate was separated by 4-20% Tris Glycine SDS-PAGE. After blocking, the membrane was probed with the primary antibody diluted to 1:1500 in 5% BLOTTO/PBS overnight at 4°C. The membrane was washed and reacted with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers.

Quick Database Links

Gene Symbol

PPP1R13L

UniProt

Q8WUF5

RefSeq:63003907