Huperzine A

SKU: orb1304611

Description

(-)-Huperzine A is a potent, selective, and reversible acetylcholinesterase inhibitor derived from the Chinese herb Huperzia serrata. It is widely used in Alzheimer's disease research, with applications in both in vitro enzymatic assays and in vivo cognitive function studies.

Research Area

Cell Biology, Neuroscience

Key Properties

• CAS Number: 102518-79-6
• MW: 242.3
• Purity: >98%
• Formula: C₁₅H₁₈N₂O
• SMILES: [H][C@@]12Cc3[nH]c(=O)ccc3[C@@](N)(CC(C)=C1)C2=CC
• Target: Acetylcholinesterase/Butyrylcholinesterase
• Solubility: Soluble in DMSO (up to 20 mg/ml)

Bioactivity

• Target IC50:
  AChE (G4 form):7 nM(Ki)
• In Vivo:
  Compared to tacrine, strychnine, galantamine, and carbachol, (-)-Huperzine A exhibits stronger inhibition of acetylcholinesterase (AChE) activity but has the least effect on butyrylcholinesterase (BuChE). (-)-Huperzine A protects cells against glutamate, hydrogen peroxide, β-amyloid, ischemia, and astrosporine-induced cytotoxicity and apoptosis. This protective function is achieved through a series of processes including the regulation of apoptotic protein expression (such as Bcl-2, Bax, p53, and caspase-3), alleviation of oxidative stress, mitochondrial protection, upregulation of nerve growth factor and its receptors, and interference with amyloid precursor protein metabolism. Notably, (-)-Huperzine A preferentially inhibits the tetrameric form of AChE (G4 form).
• In Vitro:
  In both animal models and patients with Alzheimer's Disease (AD), (-)-Huperzine A has been shown to improve learning and memory impairments. Compared to the control group treated with saline, significant inhibition of acetylcholinesterase (AchE) activity was observed in the cortex, striatum, hippocampus, medulla, septal nuclei, cerebellum, and thalamus of rats 30 minutes post-mortem.

Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Selagine; (-)-Hupezine A

Compound Identifiers

PubChem CID

449069