Human WAP Four Disulfide Core Domain Protein 2 (WFDC2) ELISA Kit

SKU: orb778704

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to WAP Four Disulfide Core Domain Protein 2(WFDC2).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human WFDC2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human WFDC2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human WFDC2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human WFDC2 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: Serum, plasma, tissue homogenates, saliva and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.32-20 ng/mL
• Sensitivity: 0.129 ng/mL
• Concentration: 20 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

WFDC2 ELISA Kit, HE4 ELISA Kit, WAP5 ELISA Kit, EDDM4 ELISA Kit, Epididymal Protein 4 ELISA Kit, Epididymal Secretory Protein E4 ELISA Kit, Putative Protease Inhibitor WAP5 ELISA Kit, Major epididymis-specific protein E4 ELISA Kit

Quick Database Links

UniProt

Q14508