Human Epididymal Protein 4 (HE4) EasyStep ELISA Kit

• Catalog Number: orb1950187
• Category: Assays and Kits
• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human HE4, and the Human HE4 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human HE4. After TMB substrate solution is added, only those wells that contain Human HE4 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HE4 in the samples is then determined by comparing the OD of the samples to the standard curve.
• Reactivity: Human
• Concentration: 10 ng/mL
• UniProt ID: Q14508
• Application notes: standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human HE4, and the Human HE4 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human HE4. After TMB substrate solution is added, only those wells that contain Human HE4 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HE4 in the samples is then determined by comparing the OD of the samples to the standard curve
• Assay Type: Sandwich
• Assay Time: 1.5h
• Range: 0.16-10 ng/mL
• Sensitivity: 160 pg/mL
• Sample Types: Serum, plasma
• Alternative names: Epididymis Protein 4; dJ461P17.6 ; EDDM4 ; epididy
• Research Area: Cancer Biology
• Note: For research use only