Human Epididymal Protein 4 (HE4) ELISA Kit

• Catalog Number: orb782375
• Category: Assays and Kits
• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to HE4(Epididymal protein 4).
• Reactivity: Human
• Concentration: 10 ng/mL
• UniProt ID: Q14508
• Tested applications: ELISA
• Application notes: standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human HE4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HE4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human HE4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HE4 in the samples is then determined by comparing the OD of the samples to the standard curve
• Assay Time: 3.5h
• Range: 15.63-1000 ng/mL
• Sensitivity: 5.15 ng/mL
• Sample Types: serum, plasma and other biological fluids
• Alternative names: Epididymis Protein 4 ELISA Kit, dJ461P17.6 ELISA K
• Note: For research use only