Human vWF ELISA Kit Discontinued

SKU: orb779414

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Von Willebrand Factor(vWF).

Research Area

Hematology, Signal Transduction

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  Standard: 10 ng/mL. Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human vWF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human vWF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human vWF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human vWF in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: plasma
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.16-10 ng/mL
• Sensitivity: 0.056 ng/mL
• Concentration: 150 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Expiration Date: Please enquire.
• Disclaimer: For research use only

Alternative Names

F8VWF ELISA Kit, VWD ELISA Kit, von Willebrand antigen 2 ELISA Kit

Quick Database Links

UniProt

P04275

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PubMed:

Applications

ELISA

Reactivity

Human