Human Von Willebrand Factor (vWF) ELISA Kit

SKU: orb2667666

Description

Human Von Willebrand Factor (vWF) ELISA Kit

Research Area

Hematology, Signal Transduction

Images & Validation

• Application Notes:
  standard: 150 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human vWF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human vWF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human vWF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human vWF in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: plasma
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 2.34-150 ng/mL
• Sensitivity: 0.56 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

F8VWF; VWD; von Willebrand antigen 2

Quick Database Links

UniProt

P04275