Human Von Willebrand Factor Cleaving Protease (vWFCP) ELISA Kit

SKU: orb775163

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Von Willebrand Factor Cleaving Protease(vWFCP).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human vWFCP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human vWFCP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human vWFCP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human vWFCP in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: serum, plasma and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.32-20 ng/mL
• Sensitivity: 0.133 ng/mL
• Concentration: 20 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

ADAMTS13 ELISA Kit, TTP ELISA Kit, vWF-cp ELISA Kit, vWF-cleaving protease ELISA Kit, A Disintegrin And Metalloproteinase With A Thrombospondin Type 1 Motif Member 13 ELISA Kit

Quick Database Links

UniProt

Q76LX8