You have no items in your shopping cart.
Cart summary
Item 1 of 2
Item 1 of 2
Human TNF-alpha Protein, His Tag
Catalog Number: orb383503
| Catalog Number | orb383503 |
|---|---|
| Category | Proteins |
| Description | Human TNF-alpha Protein, His Tag is expressed from human 293 cells (HEK293). It contains AA Val 77 - Leu 233 (Accession # NP_000585.2). |
| Target | TNF-alpha |
| Conjugation | Unconjugated |
| Tag | C-6×His |
| Reactivity | Human |
| Form/Appearance | Powder |
| Buffer/Preservatives | PBS, pH 7.4 |
| Purity | 95% |
| Protein Sequence | NP_000585.2 |
| MW | 18.3 kDa |
| Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 18.3 kDa. The protein migrates as 17-19 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation. |
| Expression System | HEK293 |
| Endotoxins | 1.0 EU per μg |
| Biological Origin | Human |
| Biological Activity | Humira (Adalimumab) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind Human TNF-alpha Protein, His Tag with an affinity constant of 0.244 nM as determined in a SPR assay (Biacore T200) (Routinely tested). |
| Expression Region | Val 77 - Leu 233 |
| Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
| Research Area | Immunology & Inflammation |
| Note | For research use only |
| NCBI | NP_000585.2 |
| Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of Human TNF-alpha protein
Humira (Adalimumab) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind Human TNF-alpha Protein, His Tag (Cat. No. orb383503) with an affinity constant of 0.244 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
- Item 1 of 4
Human TNFa Protein, His Tag [orb689423]
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 18.1 kDa after removal of the signal peptide.
Mammalian
10 μg, 50 μg, 100 μg - Item 1 of 1
- Item 1 of 1
- Item 1 of 1
Human TNF alpha His tag protein [orb80108]
FA, HPLC, SDS-PAGE
Unconjugated
> 95.0% as determined by RP-HPLC and analysis by SDS-PAGE
10 μg, 100 μg, 500 μg 
Recombinant Human TNF alpha Protein, Avi His Tag [orb1550356]
> 95% by Tris-Bis PAGE, > 95% by SEC-HPLC
Recombinant Human TNF alpha Protein is produced by Expi293 expression system. The target protein is expressed with sequence (Val77-Leu233 ) of Human TNF alpha fused with a His Tag and Avi tag at the C-terminal.
100 μg
