Canine TNF-alpha Protein, His Tag

• Catalog Number: orb762406
• Category: Proteins
• Description: Canine TNF-alpha, His Tag is expressed from human 293 cells (HEK293). It contains AA Val 77 - Leu 233 (Accession # P51742-1).
• Target: TNF-alpha
• Conjugation: Unconjugated
• Tag: C-6×His
• Reactivity: Canine
• Form/Appearance: Powder
• Buffer/Preservatives: PBS, pH 7.4
• Purity: 90%
• Protein Sequence: NP_001003244.4
• UniProt ID: P51742
• MW: 18.6 kDa
• Application notes: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 18.6 kDa. The protein migrates as 19-22 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expression System: HEK293
• Endotoxins: 1.0 EU per μg
• Biological Origin: Canine
• Biological Activity: Immobilized Humira at 5 μg/mL (100 μL/well) can bind Canine TNF-alpha, His Tag (Cat. No. ) with a linear range of 0.3-1 ng/mL (QC tested).
• Expression Region: Val 77 - Leu 233
• Storage: -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
• Note: For research use only
• NCBI: NP_001003244.4

Immobilized Humira at 5 μg/mL (100 μL/well) can bind Canine TNF-alpha, His Tag (Cat. No. orb762406) with a linear range of 0.3-1 ng/mL (QC tested).