TIGIT Mouse Monoclonal Antibody

SKU: orb2974528

Description

Anti-Human TIGIT Antibody (BUCC3629) is a mouse monoclonal antibody detecting VSTM3 in FCM. Suitable for Human. Highlights Flow Cytometry: Validated for flow cytometry applications.

Images & Validation

• Tested Applications: FC
• Reactivity: Human

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a, kappa
• Clone No.: BUCC3629
• Purity: >95% as determined by SDS-PAGE.
• Purification: Protein A/G purified from cell culture supernatant.
• Endotoxin: Please contact with the lab for this information.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M PBS, pH 7.4, 0.09% Sodium azide.
• Concentration: Lot dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

VSTM3, TIGIT, V-set and transmembrane domain-containing protein 3, VSIG9, V-set and immunoglobulin domain-containing protein 9, T-cell immunoreceptor with Ig and ITIM domains

Flow-cytometry using anti-human TIGIT antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human TIGIT monoclonal antibody (Catalog: orb2974528) at a concentration of 5 µg/mL for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC and cells analysed on a NovoCyte Flow Cytometer.

Flow-cytometry using anti-human TIGIT antibody. TIGIT Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human TIGIT antibody monoclonal antibody (Catalog # orb2974528, Green Histogram) at a concentration of 5 µg/mL for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody and cells analysed on a NovoCyte Flow Cytometer.

Quick Database Links

UniProt

Q495A1