Anti-TIGIT [1B4]

• Catalog Number: orb613975
• Category: Antibodies
• Description: Mouse monoclonal antibody to TIGIT
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 1B4
• Tested applications: Blocking, ELISA, FC
• Reactivity: Mouse
• Isotype: IgG1
• Immunogen: This antibody was raised by immunising TIGIT-/- mice with recombinant murine TIGIT tetramers.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: TIGIT
• UniProt ID: P86176
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: VSTM3; T cell Ig and ITIM domain; T cell immunorec
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Binding curve of anti-TIGIT antibody 1B4 (orb613976) to recombinant mouse TIGIT Fc-Fusion Protein. ELISA Plate coated with recombinant mouse recombinant mouse TIGIT Fc-Fusion Protein at a concentration of 2 µg/ml. A 4-fold serial dilution from 5000 ng/ml was performed using orb613976. For detection, a 1:4000 dilution of HRP-labelled anti-rabbit antibody was used.

Western Blot using anti-TIGIT antibody 1B4 RAW-264.7 cells (A), mouse spleen (B) and mouse thymus (C) lysates (35 µg protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit IgG version of 1B4 (orb613976) at 0.3 µg/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.