Human SMAD3 protein

• Catalog Number: orb707052
• Category: Proteins
• Description: Recombinant Human SMAD3 Protein is produced by E.coli expression system and the target gene encoding Ser2-Ser425 is expressed with a 6His, Flag tag at the N-terminus.
• Tested applications: ELISA, MS, SDS-PAGE, WB
• Reactivity: Human
• Form/Appearance: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 500mM NaCl, pH 7.5.
• Purity: Greater than 95% as determined by reducing SDS-PAGE.
• Target: SMAD3
• Entrez: 4088
• UniProt ID: P84022
• Protein Sequence: MRGSHHHHHH GSDYKDDDDK SSILPFTPPI VKRLLGWKKG EQNGQEEKWC EKAVKSLVKK LKKTGQLDEL EKAITTQNVN TKCITIPRSL DGRLQVSHRK GLPHVIYCRL WRWPDLHSHH ELRAMELCEF AFNMKKDEVC VNPYHYQRVE TPVLPPVLVP RHTEIPAEFP PLDDYSHSIP ENTNFPAGIE PQSNIPETPP PGYLSEDGET SDHQMNHSMD AGSPNLSPNP MSPAHNNLDL QPVTYCEPAF WCSISYYELN QRVGETFHAS QPSMTVDGFT DPSNSERFCL GLLSNVNRNA AVELTRRHIG RGVRLYYIGG EVFAECLSDS AIFVQSPNCN QRYGWHPATV CKIPPGCNLK IFNNQEFAAL LAQSVNQGFE AVYQLTRMCT IRMSFVKGWG AEYRRQTVTS TPCWIELHLN GPLQWLDKVL TQMGSPSIRC SSVS
• Source: E. coli
• Endotoxins: Endotoxin: Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.
• Storage: Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
• Buffer/Preservatives: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 500mM NaCl, pH 7.5.
• Alternative names: MADH3 protein, Mothers against decapentaplegic hom
• Note: For research use only
• Application notes: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Expiration Date: 6 months from date of receipt.