ALB Antibody

SKU: orb2652785

Description

ALB Antibody

Images & Validation

• Tested Applications: DOT, ELISA, IHC, WB
• Dilution Range: ELISA: 1:50,000 - 1:200,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Reactivity: Human
• Application Notes:
  Anti-Human Serum Albumin Antibody has been tested by ELISA, dot blot, and western blot. This product is suitable for the detection of human serum albumin in blood and tissue samples. Antibody is suitable for western blotting to detect a single band of 67 kDa, the expected apparent molecular weight, and for use in ELISA. Researchers should determine optimal titers for applications that are not stated below

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Human Serum Albumin
• Target: ALB
• Purity: Anti-Human Serum Albumin Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using purified human serum albumin. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. Analysis by SDS-PAGE was used to determine that this preparation is substantially free of aggregates and shows a banding pattern consistent with purified Rabbit IgG.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-human serum albumin antibody, rabbit anti-HSA antibody, Analbuminemia antibody, rabbit anti-serum albumin antibody, rabbit anti-albumin antibody

Dot Blot of Rabbit IgG protein. Antigen: control Rabbit IgG protein. Load: 250 ng starting total load in a serial 1:1 dilution. Primary antibody: Rabbit IgG protein. Secondary antibody: Rabbit IgG Antibody Peroxidase Conjugated and Rabbit IgG Antibody DyLight™ 649 Conjugated secondary antibodies at 1:20000 for 45 min at RT. Block: orb348637 buffer overnight at 4°C.

Western Blot of human serum albumin. Lane 1: 1:400 of FBS. Lane 2: 1:1600 of FBS. Lane 3: 1:400 of normal rabbit serum. Lane 4: 1:1600 of normal rabbit serum. Lane 5: 1:400 of normal human serum. Lane 6: 1:1600 of normal human serum. Lane 7: 1:10 of human serum albumin (10 mg/ml). Lane 8: 1:40 of human serum albumin (10 mg/ml). Lane 9: molecular weight. Right Blot: Secondary only incubated for control. Primary antibody: human serum albumin at 1:400 for overnight at 4°C. Secondary antibody: Biotin conjugated rabbit anti human serum albumin with 10 mg/ml BSA added to stock was diluted 1:5000 in PBST for 2hrs at RT. DyLight 649 conjugated Streptavidin 1:50000 in PBST 1 hr RT was used as a secondary reagent. Block: 1.5% rabbit serum overnight at 4°C.

Quick Database Links

Gene Symbol

ALB

UniProt

P02768

RefSeq (Protein):NP_000468.1