Albumin Antibody

• Catalog Number: orb1239194
• Category: Antibodies
• Description: Albumin Antibody
• Species/Host: Gallus
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Predicted Reactivity: Donkey, Equine, Gallus, Porcine, Rabbit
• Reactivity: Human, Mouse, Rat
• Isotype: IgY
• Immunogen: Albumin antibody was raised against a 13 amino acid synthetic peptide near the center of human Albumin.The immunogen is located within amino acids 340 - 390 of Albumin.
• Antibody Type: Primary Antibody
• Concentration: 1 mg/mL
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 69kD Observed: 69 kD
• Target: ALB
• UniProt ID: P02768
• NCBI: P02768
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Albumin Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: Albumin Antibody: PRO0883, PRO0903, PRO1341, Serum
• Note: For research use only
• Application notes: WB: 1-2 μg/mL; IHC-P/IF: 10-20 μg/mL; IHC-P: 2-10 μg/mL. Antibody validated: Western Blot in human, mouse and rat samples; Immunofluorescence in mouse samples; Immunohistochemistry in human, mouse, and rat samples; . All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Western Blot Validation in Human Liver. Loading: 10 µg of lysates per lane. Antibodies: Albumin, orb1239194, 1 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti- chicken IgY HRP conjugate at 1:10000 dilution.

Western Blot Validation in Mouse Liver. Loading: 10 µg of lysates per lane. Antibodies: Albumin, orb1239194, 1 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti- chicken IgY HRP conjugate at 1:10000 dilution.

Western Blot Validation in Rat Liver. Loading: 10 µg of lysates per lane. Antibodies: Albumin, orb1239194, 1 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti- chicken IgY HRP conjugate at 1:10000 dilution.

Immunohistochemistry Validation of Albumin in Human Liver. Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-albumin antibody (orb1239194) at 2 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti- chicken IgY (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunohistochemistry Validation of Albumin in Mouse Liver. Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-albumin antibody (orb1239194) at 10 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti- chicken IgY (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunohistochemistry Validation of Albumin in Rat Liver. Immunohistochemical analysis of paraffin-embedded rat liver tissue using anti-albumin antibody (orb1239194) at 2 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-chicken IgY (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunofluorescence Validation of Albumin in Mouse Liver. Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse liver tissue labeling albumin with orb1239194 at 20 /mL, followed by goat anti-chicken IgY secondary antibody at 1/500 dilution (red) and DAPI staining (blue).