Human M-CSF Protein, His Tag

• Catalog Number: orb402822
• Category: Proteins
• Description: Human M-CSF, His Tag is expressed from human 293 cells (HEK293). It contains AA Glu 33 - Arg 255 (Accession # P09603-1).
• Target: M-CSF
• Conjugation: Unconjugated
• Tag: N-6×His
• Reactivity: Human
• Form/Appearance: Powder
• Buffer/Preservatives: PBS, pH 7.4
• Purity: 95%
• UniProt ID: P09603
• MW: 26.0 kDa
• Application notes: This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 26.0 kDa. The protein migrates as 36-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expression System: HEK293
• Endotoxins: 1.0 EU per μg
• Expression Region: Glu 33 - Arg 255
• Storage: -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
• Research Area: Immunology & Inflammation
• Note: For research use only
• Expiration Date: 6 months from date of receipt.

Human M-CSF, His Tag (Cat. No. orb402822) captured on CM5 Chip via anti-His antibody can bind Human M-CSF R, Fc Tag, low endotoxin (Cat. No. orb257372) with an affinity constant of 1.9 nM as determined in SPR assay (Biacore T200) (Routinely tested).