Human M-CSF R Protein, His Tag

• Catalog Number: orb257371
• Category: Proteins
• Description: Human M-CSF R, His Tag is expressed from human 293 cells (HEK293). It contains AA Ile 20 - Glu 512 (Accession # NP_005202.2).
• Target: M-CSF R
• Conjugation: Unconjugated
• Tag: C-6×His
• Reactivity: Human
• Form/Appearance: Powder
• Buffer/Preservatives: PBS, pH 7.4
• Purity: 96%
• Protein Sequence: NP_005202.2
• UniProt ID: P07333
• MW: 55.3 kDa
• Application notes: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 55.3 kDa. The protein migrates as 80-95 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expression System: HEK293
• Endotoxins: 1.0 EU per μg
• Biological Origin: Human
• Biological Activity: Biotinylated Human M-CSF, His, (Cat. No. MCF-H82E6) immobilized on SA Chip can bind Human M-CSF R, His Tag (Cat. No. ) with an affinity constant of 0.309 μM as determined in a SPR assay (Biacore T200) (Routinely tested).
• Expression Region: Ile 20 - Glu 512
• Storage: -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
• Note: For research use only
• NCBI: NP_005202.2
• Expiration Date: 6 months from date of receipt.

SDS-PAGE analysis of Human M-CSF R protein

Biotinylated Human M-CSF, His, Avitag immobilized on SA Chip can bind Human M-CSF R, His Tag (Cat. No. orb257371) with an affinity constant of 0.309 μM as determined in a SPR assay (Biacore T200) (Routinely tested).