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Catalog Number | orb383507 |
---|---|
Category | Proteins |
Description | Mouse M-CSF R, His Tag is expressed from human 293 cells (HEK293). It contains AA Ala 20 - Ser 511 (Accession # NP_001032948). |
Target | M-CSF R |
Conjugation | Unconjugated |
Tag | C-10×His |
Reactivity | Mouse |
Form/Appearance | Powder |
Buffer/Preservatives | PBS, pH 7.4 |
Purity | 95% |
Protein Sequence | NP_001032948 |
UniProt ID | P09581 |
MW | 57.1 kDa |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 57.1 kDa. The protein migrates as 66-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expression System | HEK293 |
Endotoxins | 1.0 EU per μg |
Biological Origin | Mouse |
Biological Activity | Immobilized Mouse M-CSF Protein at 5 μg/mL (100 μL/well) can bind Mouse M-CSF R, His Tag (Cat. No. ) with a linear range of 0.078-0.625 μg/mL (Routinely tested). |
Expression Region | Ala 20 - Ser 511 |
Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
Note | For research use only |
NCBI | NP_001032948 |
Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of Mouse M-CSF protein
Immobilized Mouse M-CSF Protein at 5 μg/mL (100 μL/well) can bind Mouse M-CSF R, His Tag (Cat. No. orb383507) with a linear range of 0.078-0.625 μg/mL (Routinely tested).
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 56kDa after removal of the signal peptide. The apparent molecular mass of mCSF1R-His is approximately 70-100 kDa due to glycosylation. | |
Mammalian |