You have no items in your shopping cart.
Cart summary
Item 1 of 1
Human Latent TGF-beta 1 Protein, His Tag
Catalog Number: orb383501
| Catalog Number | orb383501 |
|---|---|
| Category | Proteins |
| Description | Human Latent TGFB1, His Tag is expressed from human 293 cells (HEK293). It contains AA Leu 30 - Ser 390 (Accession # P01137-1). |
| Target | Latent TGF-beta 1 |
| Conjugation | Unconjugated |
| Tag | N-10×His |
| Reactivity | Human |
| Form/Appearance | Powder |
| Buffer/Preservatives | PBS, pH 7.4 |
| Purity | 95% |
| Protein Sequence | NP_000651.3 |
| UniProt ID | P01137 |
| MW | 42.6 kDa (monomer) |
| Application notes | This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 42.6 kDa (monomer). The protein migrates as 38-43kDa and 50 kDa (LAP) and 13 kDa (TGFB1) under reducing (R) condition (SDS-PAGE) due to glycosylation and Interchain disulfide bond. |
| Expression System | HEK293 |
| Endotoxins | 1.0 EU per μg |
| Biological Origin | Human |
| Expression Region | Leu 30 - Ser 390 |
| Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
| Note | For research use only |
| NCBI | NP_000651.3 |
SDS-PAGE analysis of Human TGF-beta 1 protein
Latent TGF beta 1 & GARP Heterotrimer Protein, Human, Recombinant (His) [orb1953587]
98.00%
108.72 kDa (predicted); (72.33, 40.89 and 13.99) kDa (reducing conditions)
1 mg, 100 μgLatent TGF beta 1 Protein, Human, Recombinant (His), Biotinylated [orb1954345]
98.00%
42.4 kDa (predicted)
100 μg, 20 μgLatent TGF beta 3 Protein, Human, Recombinant (His) [orb1960626]
98.00%
45.9 kDa (predicted). Due to enzyme lysis and glycosylation, the protein migrates to 46-50 kDa and 55-60 kDa based on Tris-Bis PAGE result.
1 mg, 500 μg, 100 μgLatent TGF beta 1 Protein, Human, Recombinant (His) [orb1958487]
99.50%
42.4 kDa (predicted); 16, 38 and 55 kDa (reducing condition, due to glycosylation); 110 kDa (non-reduced condition, due to glycosylation)
50 μg, 100 μgRecombinant Human Latent TGF beta 1(C33S) Protein, His Tag [orb1550642]
> 95%by Bis-Tris PAGE
Recombinant Human Latent TGF beta 1(C33S) Protein is produced by HEK293 Cells expression system. The target protein is expressed with sequence (Leu30-Ser390���C33S mutant���) of human Latent TGF beta 1(C33S) (Accession #) fused with a His Tag at the N-terminal and Avi Tag at the C-terminal.
100 μg
