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Catalog Number | orb867322 |
---|---|
Category | Proteins |
Description | Human Latent TGF-beta 3, His Tag is expressed from human 293 cells (HEK293). It contains AA Leu 24 - Ser 412 (Accession # P10600-1). |
Reactivity | Human |
Tag | N-10×His |
Form/Appearance | Powder |
Purity | 90% |
Conjugation | Unconjugated |
MW | 46.8 kDa |
Target | TGF-beta 3 |
UniProt ID | P10600 |
Protein Sequence | NP_003230.1 |
Expression System | HEK293 |
Biological Origin | Human |
Expression Region | Leu 24 - Ser 412 |
Endotoxins | 1.0 EU per μg |
NCBI | NP_003230.1 |
Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
Buffer/Preservatives | PBS, pH 7.4 |
Note | For research use only |
Application notes | This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 46.8 kDa. The protein migrates as 12 kDa and 40-43 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
98.00% | |
45.9 kDa (predicted). Due to enzyme lysis and glycosylation, the protein migrates to 46-50 kDa and 55-60 kDa based on Tris-Bis PAGE result. |
98.00% | |
12&37-45 KDa (reducing condition) |
Greater than 90.0% as determined by SDS-PAGE. | |
Sf9, Baculovirus cells |