Human IL-5 Protein, His Tag

• Catalog Number: orb612148
• Category: Proteins
• Description: Human IL-5, His Tag, premium grade is expressed from human 293 cells (HEK293). It contains AA Ile 20 - Ser 134 (Accession # P05113-1).
• Target: IL-5
• Conjugation: Unconjugated
• Tag: N-10×His
• Reactivity: Human
• Form/Appearance: Powder
• Buffer/Preservatives: PBS, pH 7.4
• Purity: 90%
• UniProt ID: P05113
• MW: 15.0 kDa
• Application notes: This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 15.0 kDa. The protein migrates as 18-23 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expression System: HEK293
• Endotoxins: 0.1 EU per μg
• Expression Region: Ile 20 - Ser 134
• Storage: -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
• Research Area: Immunology & Inflammation
• Note: For research use only
• Expiration Date: 6 months from date of receipt.

Loaded Human IL-5, His Tag, premium grade (Cat. No. orb612148) on NTA Biosensor, can bind Human IL-5 R alpha, Fc Tag with an affinity constant of 4.63 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).