Human IL5 Protein, His Tag

• Catalog Number: orb689428
• Category: Proteins
• Description: Recombinant Human IL5 protein with C-terminal 6×His tag
• Target: IL5
• Tag: C-6×His Tag
• Reactivity: Human
• Form/Appearance: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
• Buffer/Preservatives: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions.
• Purity: The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
• Purification: Purified from cell culture supernatant by affinity chromatography
• UniProt ID: P05113
• MW: The protein has a predicted molecular mass of 13.94 kDa after removal of the signal peptide.The apparent molecular mass of IL5-His is approximately 15-25 kDa due to glycosylation.
• Source: Mammalian
• Storage: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
• Alternative names: IL-5;TRF;Interleukin-5;EDF
• Research Area: Epigenetics
• Note: For research use only
• Expiration Date: 6 months from date of receipt.

Human CD30 Protein, His Tag on SDS-PAGE under reducing condition.

ELISA plate pre-coated by 2 μg/ml (100 μL/well) Human CD30, His tagged protein (orb689465) can bind Anti-CD30 Neutralizing antibody orb689322 in a linear range of 0.13-80.0 ng/ml.

ELISA plate pre-coated by 2 μg/ml (100 μL/well) Human CD30, His tagged protein (orb689465) can bind Human CD30 Ligand, mFc-His tagged protein orb689400 in a linear range of 2.56-320 ng/ml.

Flow cytometry analysis with 1 μg/mL Human CD30 Protein, His tag (orb689465) on Expi293 cells transfected with human CD30L (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).