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| Catalog Number | orb689433 |
|---|---|
| Category | Proteins |
| Description | Recombinant human L5RA protein with C-terminal 6×His tag |
| Target | IL5RA |
| Tag | C-6×His Tag |
| Reactivity | Virus |
| Form/Appearance | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| Buffer/Preservatives | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions. |
| Purity | The purity of the protein is greater than 90% as determined by SDS-PAGE and Coomassie blue staining. |
| Purification | Purified from cell culture supernatant by affinity chromatography |
| UniProt ID | Q01344 |
| MW | The protein has a predicted molecular mass of 37.0 kDa after removal of the signal peptide.The apparent molecular mass of IL5RA-His is approximately 50-55 kDa due to glycosylation. |
| Source | Mammalian |
| Storage | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| Alternative names | IL5Ra;CD125;IL-5 R alpha |
| Research Area | Epigenetics |
| Note | For research use only |

SARS-CoV-2 (2019-nCoV) S protein RBD, mFc Tag on SDS-PAGE under reducing condition.

ELISA plate pre-coated by 2 μg/ml (100 μL/well) S-RBD, mFc tagged protein (orb689474) can bind Human ACE2, hFc Tagged protein orb689421 in a linear range of 7.81-87.7 ng/ml.
> 90% by SDS-PAGE. | |
Recombinant Human IL5RA Protein is produced by HEK293 Cells expression system. The target protein is expressed with sequence (IL5RA(Asp21-Pro338)) of human IL5RA (Accession #) fused with a His Tag at the C-terminus. |
>95%, determined by SDS-PAGE | |
This protein contains the human IL5Ra isoform 2 (Q01344-2) (Met 1-Arg 335) was fused with a polyhistidine tag at the C-terminus and expressed from HEK293 Cells. |
>90% as determined by SDS-PAGE. | |
18.31 kDa |
>80% as determined by SDS-PAGE. | |
47.69 kDa |
>85%, determined by SDS-PAGE | |
This protein contains the human IL5Ra isoform 2 (Q01344-2) (Met 1-Arg 335) was fused with a polyhistidine tag at the C-terminus and expressed from HEK293 Cells. |
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