Human IGF-I R Protein, His Tag

• Catalog Number: orb511230
• Category: Proteins
• Description: Human IGF-I R, His Tag is expressed from human 293 cells (HEK293). It contains AA Glu 31 - Asn 932 (Accession # P08069-1).
• Target: IGF-I R / IGF-1 R
• Conjugation: Unconjugated
• Tag: C-10×His
• Reactivity: Human
• Form/Appearance: Powder
• Buffer/Preservatives: PBS, pH 7.4
• Purity: 90%
• UniProt ID: P08069
• MW: 104.8 kDa
• Application notes: This protein carries a polyhistidine tag at the C-terminus. This protein contains a furin convertase cleavage site, 737-RKRR-740, and will be partially processed into N (α chain) and C-terminal fragment (partial β chain) with calculated MW of 81.0 kDa and 23.8 kDa respectively. The protein migrates as 35-45 kDa, 110-120 kDa and 130 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expression System: HEK293
• Endotoxins: 1.0 EU per μg
• Expression Region: Glu 31 - Asn 932
• Storage: -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
• Research Area: Disease Biomarkers
• Note: For research use only
• Expiration Date: 6 months from date of receipt.

Human IGF-I R, His Tag (Cat. No. orb511230) immobilized on CM5 Chip can bind Biotinylated Human IGF-I, His, Avitag with an affinity constant of 68 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).