Mouse IGF-I R Protein, His Tag

• Catalog Number: orb511248
• Category: Proteins
• Description: Mouse IGF-I R, His Tag is expressed from human 293 cells (HEK293). It contains AA Glu 31 - His 936 (Accession # Q60751-1).
• Target: IGF-I R
• Conjugation: Unconjugated
• Tag: C-10×His
• Reactivity: Mouse
• Form/Appearance: Powder
• Buffer/Preservatives: PBS, pH 7.4
• Purity: 90%
• Protein Sequence: NP_034643.2
• UniProt ID: Q60751
• MW: 81.7 kDa, 24.3 kDa
• Application notes: This protein carries a polyhistidine tag at the C-terminus. This protein contains a furin convertase cleavage site, 738-RRRR-741, and will be partially processed into N (α chain) and C-terminal fragment (partial β chain) with calculated MW of 81.7 kDa and 24.3 kDa respectively. The protein migrates as 45-55 kDa (partial β chain), 100-125 kDa (α chain) and 150 kDa (α chain & partial β chain) under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expression System: HEK293
• Endotoxins: 1.0 EU per μg
• Biological Origin: Mouse
• Biological Activity: Immobilized Mouse IGF-I R, His Tag (Cat. No. ) at 5 μg/mL (100 μL/well) can bind Human IGF-I, Fc Tag (Cat. No. IG1-H4269) with a linear range of 7.8-125 ng/mL (QC tested).
• Expression Region: Glu 31 - His 936
• Storage: -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
• Note: For research use only
• NCBI: NP_034643.2

Line graph illustrates about the Ag-Ab reactions using different concentrations of antigen and serial dilutions of Mouse IGF-I R / CD221 Protein.

SDS-Page analysis of Mouse IGF-I R / CD221 Protein.

Immobilized Mouse IGF-I R, His Tag (Cat. No. orb511248) at 5 μg/mL (100 μL/well) can bind Human IGF-I, Fc Tag (Cat. No. orb257564) with a linear range of 7.8-125 ng/mL (QC tested).