Rat IGF-I R Protein, His Tag

SKU: orb511247

Description

Rat IGF-I R, His Tag is expressed from human 293 cells (HEK293). It contains AA Glu 31 - His 936 (Accession # P24062-1).

Images & Validation

• Application Notes:
  This protein carries a polyhistidine tag at the C-terminus. This protein contains a furin convertase cleavage site, 738-RRRR-741, and will be partially processed into N (α chain) and C-terminal fragment (partial β chain) with calculated MW of 81.3 kDa and 24.2 kDa respectively. The protein migrates as 45-50 kDa (partial β chain), 96-115 kDa (α chain) and 120 kDa (α chain & partial β chain) under reducing (R) condition (SDS-PAGE) due to glycosylation.

Key Properties

• Expression System: HEK293
• Target: IGF-I R / IGF-1 R
• Reactivity: Rat
• Tag: C-10×His
• Molecular Weight: 105.5 kDa
• Expression Region: Glu 31 - His 936
• Purity: 90%
• Endotoxins: 1.0 EU per μg
• Conjugation: Unconjugated

Storage & Handling

• Storage: -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
• Form/Appearance: Powder
• Buffer/Preservatives: PBS, pH 7.4
• Disclaimer: For research use only

Immobilized Rat IGF-I R, His Tag (Cat. No. orb511247) at 5 μg/mL (100 μL/well) can bind Human IGF-I, Fc Tag (Cat. No. orb257564) with a linear range of 5-39 ng/mL (QC tested).

Quick Database Links

Gene Symbol

IGF-I R / IGF-1 R

UniProt

P24062