HRAS Mouse Monoclonal Antibody

SKU: orb2957424

Description

Anti-Human HRAS Antibody (BA35X471) is a mouse monoclonal antibody detecting c-H-ras in IF, IP, WB. Suitable for Human. Highlights Multi-Application: Validated across multiple applications.

Research Area

Signal Transduction

Images & Validation

• Tested Applications: IF, IP, WB
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a, kappa
• Clone No.: BA35X471
• Purity: >95% as determined by SDS-PAGE.
• Purification: Protein A/G purified from cell culture supernatant.
• Endotoxin: Please contact with the lab for this information.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M PBS, pH 7.4.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

c-H-ras, GTPase HRas, H-Ras-1, p21ras, Transforming protein p21, Ha-Ras, HRAS1, HRAS

Flow-cytometry using FITC anti-human HRAS antibody. MCF-7 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human HRAS monoclonal antibody (Catalog: orb2957424, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.

SDS-PAGE for Anti-Human HRAS Antibody.

Western blot analysis was performed using anti-KRAS/K-Ras 2 monoclonal antibody at 1 µg/mL on various samples. Lane 1: recombinant human KRAS/K-Ras 2 (Catalog No: orb2964027), 50 ng, Lane 2: recombinant human KRAS/K-Ras 2 (Catalog No: orb2964027), 30 ng.

Quick Database Links

UniProt

P01112