KRAS+HRAS+NRAS/KRAS Rabbit Monoclonal Antibody

SKU: orb547055

Description

Anti-KRAS+HRAS+NRAS Rabbit Monoclonal Antibody. Tested in WB, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Research Area

Cancer Biology, Epigenetics & Chromatin, Signal Transduction

Images & Validation

• Tested Applications: FC, ICC, IF, IP, WB
• Dilution Range: WB 1:500-2000 ICC/IF 1:50-200 IP 1:20 FC 1:20
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone No.: EBE-14
• Immunogen: A synthesized peptide derived from human KRAS+HRAS+NRAS
• Molecular Weight: 21 kDa
• Purification: Affinity-chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. *This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
• Concentration: 0.5mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

GTPase NRas; Transforming protein N-Ras; NRAS; HRAS1

KRAS+HRAS+NRAS was immunoprecipitated from 1mg of A549 whole cell lysate. A549 whole cell lysate 10ug (Input). The second antibody be without interference from denatured IgG.

WB analysis of KRAS+HRAS+NRAS expression in (1) 293T Cell; (2) C6 Cell.

Immunofluorescent analysis of HeLa cells, using KRAS+HRAS+NRAS Antibody.

Quick Database Links

UniProt

P01116, P01112, P01111