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Catalog Number | orb547055 |
---|---|
Category | Antibodies |
Description | Anti-KRAS+HRAS+NRAS Rabbit Monoclonal Antibody. Tested in WB, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. |
Clonality | Monoclonal |
Species/Host | Rabbit |
Isotype | Rabbit IgG |
Conjugation | Unconjugated |
Reactivity | Human, Mouse, Rat |
Form/Appearance | Liquid |
Concentration | Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure. |
Immunogen | A synthesized peptide derived from human KRAS+HRAS+NRAS |
UniProt ID | P01116, P01112, P01111 |
MW | 21 kDa |
Tested applications | FC, ICC, IF, IP, WB |
Application notes | WB 1:500-1:2000ICC/IF 1:50-1:200IP 1:50FC 1:200. Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL |
Antibody Type | Primary Antibody |
Clone Number | EBE-14 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Alternative names | GTPase NRas; Transforming protein N-Ras; NRAS; HRA Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
KRAS+HRAS+NRAS was immunoprecipitated from 1mg of A549 whole cell lysate. A549 whole cell lysate 10ug (Input). The second antibody be without interference from denatured IgG.
WB analysis of KRAS+HRAS+NRAS expression in (1) 293T Cell; (2) C6 Cell.
Immunofluorescent analysis of HeLa cells, using KRAS+HRAS+NRAS Antibody.