Human Collagen Type VI protein

• Catalog Number: orb750386
• Category: Proteins
• Description: Human Collagen Type VI protein
• Target: COL6A1-COL6A3
• Conjugation: Unconjugated
• Form/Appearance: Liquid (sterile filtered)
• Concentration: 1.0 mg/mL
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Purity: Human Collagen type VI has been prepared from Human Placenta and is chromatographically and immunologically pure. This product is free from other collagens, human serum proteins and non-collagen extracellular matrix proteins. This product reacts with anti-Collagen Type VI. Reaction with Rockland’s anti-Collagen I, II, III, IV or V is negligible (typically less than 1% cross reactivity was detected by ELISA).
• UniProt ID: P12109
• Tested applications: ELISA, IHC, SDS-PAGE, WB
• Dilution range: IF: 1:100-1:500, WB: 1:1000
• Application notes: Human Collagen type VI purified protein standard is used as a control in ELISA and western blot. Collagen type VI is recognized by type specific anti-collagen antibodies that recognize a native three-dimensional structure.
• Source: Human
• Biological Origin: Human
• Storage: Store vial at 4° C prior to opening. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Alternative names: Type VI collagen protein, collagen 6 protein, huma
• Note: For research use only
• NCBI: NP_001839.2

COL6 enhances lung epithelial cell wound repair.(a) Representative images of wound-healing response for 16HBE cells plated on COL6, Matrigel, or COL1. (b-c) Quantitation of wound width at 10 hr post-injury (relative to 0 hr) for 16HBE(b) and NHBE(c) plated on individual matrices, or combinations of matrices. N = 9, ** P < 0.01, *** P < 0.001.

COL6 promotes lung epithelial cell spreading.(a) Representative images of initial, post-adhesion spreading for 16HBE human lung epithelial cells 3 hours after plating cells on COL6, COL1 or Matrigel. Arrows show examples of spread cells. (b-d) Quantitation of the percentage of cells spread 3 hours after plating on different matrices for 16HBE (b), NHBE (c), or PHLE (d) cells. N = 6.* P < 0.05, ** P < 0.01, *** P < 0.001.

COL6-mediated spreading requires ITGB1, not NG2.(a) Representative images of untreated 16HBE cells, and 16HBE treated with anti-Integrin β1 or anti-NG2 antibodies 3 hours after plating on COL6. (b) Quantitation of untreated and anti-ITGB1 (b) or anti-NG2 (c) treated 16HBE spreading 3 hours after plating on COL6, COL1, Matrigel coated wells, and uncoated tissue-culture wells (n = 6). * P < 0.05, ** P < 0.01, *** P < 0.001.

Dot Blot results using Rabbit Anti-Collagen VI Antibody. Sample: A) Human Collagen VI (p/n orb750386), B) Bovine/Human Collagen VI (p/n orb750380). Load: 1) 1000 ng, 2) 333.33 ng, 3) 111.11 ng, 4) 37.03 ng, 5) 12.35 ng. Primary Antibody: Rabbit Anti-Collagen VI antibody at 5 µg/ml at RT for 1hr. Secondary Antibody: Goat Anti-Rabbit IgG HRP Conjugated (p/n orb347673) 1:40000 at RT for 30 mins. Blocking: BlockOut® Universal Blocking Buffer (p/n orb348644) at RT for 30 mins.

Fourier-transform infrared (FTIR) spectroscopy. Mean FTIR spectra of the five collagen types. Maximal intensity.

FTIR spectra classification model for the five collagen types. n = 30 spectra/collagen type; four spectral intervals, 1, 700–1, 600 cm−1, 1, 480– 1, 350 cm−1, 1, 300–1, 180 cm−1, and 1, 100–1, 005 cm−1. h intracluster heterogeneity, H intercluster heterogeneity.

SDS-PAGE results of Human Collagen Type VI. Lane: 1) Opal prestain molecular weight marker, 2) blank, 3) Human Collagen Type VI (p/n orb750386) [10 µg]. Coomassie stained.

Western blot results using Rabbit Anti-Collagen Type VI Antibody. Lane: 1) Human Collagen Type VI (p/n orb750386) reduced and boiled, 3) Human Collagen Type VI reduced and not boiled, 5) Opal pre-stained molecular weight marker, 7) Human Collagen Type VI not reduced and boiled, 9) Human Collagen Type VI not reduced and not boiled. Lanes 2, 4, 6, 8 blank. Load: 10 µg. Primary Antibody: Rabbit Anti-Collagen Type VI Antibody 1:500 overnight at 2-8°C. Secondary Antibody: Goat anti-Rabbit IgG HRP conjugated (p/n orb347673) 1:40000 at RT for 30 mins. Blocking: BlockOut® Universal Blocking Buffer (p/n orb348644) at RT for 1hr.