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| Catalog Number | orb1172664 |
|---|---|
| Category | Antibodies |
| Description | Human CD30 Mouse Monoclonal Antibody |
| Target | TNFRSF8 |
| Clonality | Monoclonal |
| Species/Host | Mouse |
| Isotype | Mouse IgG1, k |
| Conjugation | Unconjugated |
| Reactivity | Human |
| Predicted Reactivity | Human |
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4). |
| Purification | Affinity purified by Protein G |
| MW | 105 kDa |
| Tested applications | FC |
| Dilution range | Flow-Cyt=1ug/Test |
| Antibody Type | Primary Antibody |
| Clone Number | 3C11 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | CD 30; CD30 antigen; CD30L receptor; Cytokine rece Read more... |
| Research Area | CD Markers, Cancer Research, Cell Markers, Immunol Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |

Blank control (black line): SR. Primary Antibody (green line): Mouse Anti-human CD30 antibody (orb1172664), Dilution: 1 ug/Test, Secondary Antibody (white blue line): Goat anti-mouse IgG-FITC, Dilution: 0.5 ug/Test. Isotype control (orange line): Normal Mouse IgG, Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.
FC | |
Human | |
Human | |
Mouse | |
Monoclonal | |
PE |
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