CD30/TNFRSF8 Mouse Monoclonal Antibody

SKU: orb2974558

Description

CD30/TNFRSF8 Mouse Monoclonal Antibody is an unconjugated monoclonal antibody. It shows reactivity with Human samples. It is suitable for use in FC application. The antibody is supplied in liquid form. It is Protein A/G purified from cell culture supernatant. Purity is >95% as determined by SDS-PAGE.

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: FC
• Reactivity: Human

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone No.: BUCC3512
• Purity: >95% as determined by SDS-PAGE.
• Purification: Protein A/G purified from cell culture supernatant.
• Endotoxin: Please contact with the lab for this information.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M PBS, pH 7.4, 0.09% Sodium azide.
• Concentration: Lot dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CD30L receptor, Ki-1 antigen, CD30, TNFRSF8, D1S166E, Tumor necrosis factor receptor superfamily member 8, Lymphocyte activation antigen CD30

Flow-cytometry using anti-human CD30 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human CD30 monoclonal antibody (Catalog: orb2974558) at a concentration of 5 µg/mL for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC and cells analysed on a NovoCyte Flow Cytometer.

Flow-cytometry using anti-human CD30 antibody. CD30 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD30 antibody monoclonal antibody (Catalog # orb2974558, Green Histogram) at a concentration of 5 µg/mL for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody and cells analysed on a NovoCyte Flow Cytometer.

Flow-cytometry using anti-human CD30 antibody. Jurkat cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD30 antibody monoclonal antibody (Catalog # orb2974558, Green Histogram) at a concentration of 5 µg/mL for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody and cells analysed on a NovoCyte Flow Cytometer.

Quick Database Links

UniProt

P28908