human CD25 Antibody

SKU: orb1925419

Description

human CD25 Antibody

Images & Validation

• Tested Applications: FC
• Dilution Range: FC - 1:50
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Overlay histogram showing PHA-stimulated huuman peripheral blood lymphocytes stained with CD25 antibody (green line). The cells were icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1: 50 dilution) for 60min at 37°C. The secondary antibody used was Goat Anti-Mouse IgG, DyLight 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40min at 37°C. Isotype control antibody (blue line) was mouse IgG2a (1 μg/1x10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.

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