BAX Mouse Monoclonal Antibody

SKU: orb2959944

Description

Anti-Human BAX Antibody (B8C9) is a mouse monoclonal antibody detecting BAX in ELISA, FCM, WB. Suitable for Human and Mouse. Highlights Multi-Application: Validated across multiple applications. Flow Cytometry: Validated for flow cytometry applications.

Images & Validation

• Tested Applications: ELISA, FC, WB
• Reactivity: Human, Mouse

Key Properties

• Antibody Type: Recombinant Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone No.: B8C9
• Purity: >95% as determined by SDS-PAGE.
• Purification: Protein A/G purified from cell culture supernatant.
• Endotoxin: Please contact with the lab for this information.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M PBS, pH 7.4.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

BAX, Bcl2-L-4, BCL2L4, Bcl-2-like protein 4, Apoptosis regulator BAX

Flow-cytometry using anti-human BAX antibody. A549 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human BAX antibody monoclonal antibody (Catalog # orb2959944, Green Histogram) at a concentration of 5 µg/mL for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody and cells analysed on a NovoCyte Flow Cytometer.

Flow-cytometry using anti-human BAX antibody. HepG2 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human BAX antibody monoclonal antibody (Catalog # orb2959944, Green Histogram) at a concentration of 5 µg/mL for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody and cells analysed on a NovoCyte Flow Cytometer.

Flow-cytometry using anti-human BAX antibody. Human Jurkat cell line were stained with an irrelevant antibody (Blue Histogram) or an anti-human BAX antibody monoclonal antibody (Catalog # orb2959944, Green Histogram) at a concentration of 5 µg/mL for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody and cells analysed on a NovoCyte Flow Cytometer.

Flow-cytometry using FITC anti-human BAX antibody. TNFa stimulated U937 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human BAX monoclonal antibody (Catalog: orb2959944, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.

Flow-cytometry using PE anti-human BAX antibody. TNFa stimulated U937 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an PE anti-human BAX monoclonal antibody (Catalog: orb2959944, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.

SDS-PAGE for Anti-Human BAX Antibody.

Quick Database Links

UniProt

Q07812