Bax Mouse Monoclonal Antibody

• Catalog Number: orb500966
• Category: Antibodies
• Description: Bax Mouse Monoclonal Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 3C1
• Tested applications: ICC, IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Insect, Monkey, Yeast
• Reactivity: Human
• Isotype: IgG1
• Immunogen: KLH conjugated synthetic peptide derived from human Bax (31-130/192aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100, IF=1:200-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 21 kDa
• Target: BAX
• UniProt ID: Q07812
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: apoptosis regulator BAX; Apoptosis regulator BAX c
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraformaldehyde-fixed, paraffin embedded (Human brain glioma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Bax) Monoclonal Antibody, Unconjugated (orb500966) at 1:400 overnight at 4°C, followed by a conjugated Goat Anti-Mouse IgG antibody for 90 minutes, and DAPI for nuclei staining.

Paraformaldehyde-fixed, paraffin embedded (Human brain glioma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Bax) Monoclonal Antibody, Unconjugated (orb500966) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Sample: 293T (Human) Cell Lysate at 30 ug, 293T-UV (Human) Cell Lysate at 30 ug, Primary: Anti-Bax (orb500966) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 21 kD, Observed band size: 21 kD.

Sample: Lane 1: Human 293T cell lysates, Lane 2: Human HT-1080 cell lysates, Lane 3: Human Raji cell lysates, Lane 4: Human SH-SY5Y cell lysates, Primary: Anti-Bax (orb500966) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 21 kDa, Observed band size: 20 kDa.

Tissue/Cell: Hela cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (Bax) monoclonal Antibody, Unconjugated (orb500966) 1:100, 90 minutes at 37°C, followed by a CY3 conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.