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Catalog Number | orb344620 |
---|---|
Category | Antibodies |
Description | Hub1 antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, WB |
Reactivity | Yeast |
Isotype | IgG |
Immunogen | This purified antibody was prepared from rabbit serum after repeated immunizations with recombinant yeast Hub1 protein. |
Concentration | 5.0 mg/mL |
Dilution range | ELISA: 1:1,000 - 1:5,000, WB: 1:500 - 1:2,000 |
Form/Appearance | Lyophilized |
Purity | This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. |
Conjugation | Unconjugated |
UniProt ID | Q6Q546 |
NCBI | 6324360 |
Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Alternative names | rabbit anti-Hub1 Antibody, rabbit anti-Hub 1 Antib Read more... |
Note | For research use only |
Application notes | This purified polyclonal antibody reacts with yeast Hub1 by western blot and ELISA. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation. This antibody using the specified conditions may recognize other prominent intrinsic bands (UBLs or conjugates). Other intrinsic bands are readily detectable at lower dilutions. For immunoblotting a 9.7 kDa band corresponding to yeast Hub1 is detected. Most yeast cell lysates can be used as a positive control without induction or stimulation. |
Expiration Date | 12 months from date of receipt. |
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