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Featured
Description
Research Area
Neuroscience
Images & Validation
−Item 1 of 6
| Tested Applications | IF, IHC, WB |
|---|---|
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human HSPB1 (NP_001531.1). |
| Target | HSPB1 |
| Molecular Weight | Observed: 25kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
| Concentration | batch dependent |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−HSPB1, CMT2F, DKFZp586P1322, HS.76067, HSP27, HSP28, Hsp25, HMN2B, SRP27, HEL-S-102
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Western blot analysis of extracts from normal (control) and HSPB1 knockout (KO) HeLa cells, using HSPB1 antibody (orb1274486) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
Immunohistochemistry of paraffin-embedded human breast cancer using HSPB1 antibody (orb1274486) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat ovary using HSPB1 antibody (orb1274486) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using HSPB1 antibody (orb1274486) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using HSPB1 antibody (orb1274486) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using HSPB1 antibody (orb1274486) at dilution of 1:100. Blue: DAPI for nuclear staining.
Quick Database Links
Gene Symbol
HSPB1
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
HSPB1 Antibody (orb1274486)
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