HSP40, Hdj1 Antibody

• Catalog Number: orb1822482
• Category: Antibodies
• Description: Mouse Anti-Human HSP40, Hdj1 Monoclonal IgG1
• Target: HSP40
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG1
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS pH 7.2, 50% glycerol, 0.09% sodium azide *Storage buffer changes when conjugated
• Purification: Protein G Purified
• Immunogen: Recombinant Human Protein HSP40 (Hdj1)
• UniProt ID: P25685
• MW: 40kDa
• Tested applications: ELISA, ICC, IF, IHC, IP, WB
• Dilution range: WB (1:2000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
• Application notes: 0.5 µg/ml of SMC-145 was sufficient for detection of HSP40 (HDJ1) in 15 µg of HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
• Clone Number: 3B9.E6
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: DNAJ1 Antibody, NDAJB1 Antibody, HDJ1 Antibody, HS
• Research Area: Cancer Biology, Cell Biology, Protein Biochemistry
• Note: For research use only
• Entrez: 3337
• NCBI: NP_006136.1

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hsp40 Monoclonal Antibody, Clone 3B9.E6. Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20°C. Primary Antibody: Mouse Anti-Hsp40 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.

Western Blot analysis of Human Cell lysates showing detection of Hsp40 protein using Mouse Anti-Hsp40 Monoclonal Antibody, Clone 3B9.E6. Load: 15 μg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp40 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.