DNAJC2 Rabbit Polyclonal Antibody

• Catalog Number: orb183162
• Category: Antibodies
• Description: DNAJC2 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Equine, Gallus, Mouse, Rabbit, Sheep
• Reactivity: Human, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human DNAJC2 (521-621/621aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 72 kDa
• Target: DNAJC2
• UniProt ID: Q99543
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Zuotin-1; AU020218; DnaJ(Hsp40) homolog subfamily
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

25 ug total protein per lane of various lysates probed with DNAJC2 polyclonal antibody, unconjugated (orb183162) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (DNAJC2) Polyclonal Antibody, Unconjugated (orb183162) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (DNAJC2) Polyclonal Antibody, Unconjugated (orb183162) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Tissue/Cell: rat spleen tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-DNAJC2 Polyclonal Antibody, Unconjugated (orb183162) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.