Hsp31p Antibody

SKU: orb1248541

Description

Hsp31p Antibody

Research Area

Neuroscience

Images & Validation

• Tested Applications: ELISA, KO/KD Validated, WB
• Reactivity: Yeast
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 24kDa band observed in lysates of postdiauxic (overnight grown yeast cells, and no signal in KO grown under identical conditions. Cells grown in logarithmic phase show diminished signals as expected. (calculated MW of 25.7kDa according to NP_010822.1). Recommended concentration: 0.02-0.06µg/mL. This antibody has been successfully used in the following paper: Natkańska et al (2018) PMID: 29264711.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-ETGKFGWDEHSLAKD
• Target: Hsp31p
• Molecular Weight: Approx 24kDa
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Hsp31p, YDR533C

orb1248541 (0.05 ug/ml) staining of yeast lysate (wt) and the KO (delta) in th eleft panel and the Ponceau stain in the right panel (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Quick Database Links

Gene Symbol

Hsp31p

UniProt

Q04432

RefSeq (Protein):NP_010822.1