Hsp31p (yeast, aa50-64) Antibody

SKU: orb167681

Description

Goat polyclonal antibody to Hsp31p.

Research Area

Infectious Disease & Virology

Images & Validation

• Tested Applications: ELISA, KO/KD Validated, WB
• Dilution Range: ELISA: 1:128000, WB: 0.02-0.06 μg/ml
• Reactivity: Saccharomyces
• Predicted Reactivity: Yeast
• Application Notes:
  WB: Approx 24kDa band observed in lysates of postdiauxic (overnight grown yeast cells, and no signal in KO grown under identical conditions. Cells grown in logarithmic phase show diminished signals as expected. (calculated MW of 25.7kDa according to NP_010822

Key Properties

• Clonality: Polyclonal
• Target: Hsp31p (yeast, aa50-64)
• Protein Sequence: ETGKFGWDEHSLAKD
• Molecular Weight: 25.7
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti Hsp31p antibody, anti YDR533C antibody

Western blot analysis of yeast lysate (wt) and the KO (delta) in th eleft panel and the Ponceau stain in the right panel (35ug protein in RIPA buffer) using Hsp31p antibody

Quick Database Links

Gene Symbol

Hsp31p (yeast, aa50-64)